DATE: July 21, 2017
TIME: 10:00am PT, 1:00pm ET

CRISPR/Cas9 techniques are quickly growing in popularity for the generation of new mouse models for research. Though the approach is relatively simple, many researchers struggle with understanding the complexities associated with the diverse alleles that can be generated, how to deal with mosaicism, and how to move from a single founder mouse to larger cohorts for phenotype characterization.

Learning Objectives:

• Understanding the molecular mechanisms that drive the array of possible mutations that can result from a single Cas9/sgRNA targeting strategy
• A summary of various common targeting strategies to generate indel, knockout, and knockin mutations using CRISPR/Cas9
• Selection of founder mice and breeding strategies for mitigating mosaicism in founder mice
• Breeding strategies to establish a colony and generate cohorts for experimentation