Getting started with NGS-based immune profiling

The use of next-generation sequencing (NGS) technologies to detect B-cell receptor (BCR) or T-cell receptor (TCR) clonotypes can provide indispensable insight, but the process can be quite intimidating for new users. There are a variety of ways to set up your experiment, prepare the libraries, and analyze the data. Samples of limited input or low quality provide additional challenges.

If you are considering NGS-based immune profiling for TCR and/or BCR clonotype detection, download our new educational poster for valuable tips to ensure success. 

This poster outlines key factors to consider when designing your NGS-based immune profiling assays:

• Example applications for BCR and TCR profiling
• Tips on
  • Input selection
  • Sample isolation
  • Library prep strategy
  • Library quantitation
  • Sequencing setup
  • Data analysis

Whether you're conducting an immune profiling experiment to study immune cell development, explore autoimmune disease or cancer mechanisms, or identify new biomarkers for immune-mediated diseases, the workflow requires attention to the same key success factors.

Get started with NGS-based immune profiling!