A Guide to Single-Cell Sequencing

Single-cell sequencing is a valuable tool for analyzing complex cellular populations, enabling high-resolution views of the transcriptome, epigenome, and immune repertoire that are not possible through conventional bulk sequencing. This eBook explores the fundamentals of single-cell sequencing, focusing on how this technology works, optimized approaches to enhance results, and use-case applications to help you get the most out of your single-cell research.

What You'll Learn:

• The basics of single-cell sequencing, including the most common types of single-cell techniques and key considerations for project design
• Leveraging dead cell removal to overcome low cell viability and generate high-quality sequencing results
• Alternate cell fixation methods for preparing single-cell samples, without sacrificing integrity or data quality
• Using single-cell analysis to identify rare cells, characterize their transcriptomes, and discover potential biomarkers
• High-resolution spatial profiling and biomarker discovery using spatial transcriptomics technology