This multiplex ELISA kit for pro-inflammatory cytokines is designed for semi-quantitative and simultaneous determination of pro-inflammatory cytokines including interleukin-1_ (IL-1_), interleukin 1_ (IL-1_), interleukin-6 (IL-6), interleukin-8 (IL-8), granulocyte macrophage colony stimulating factor (GM-CSF), monocyte chemotactic and activating factor (MCAF), and tumor necrosis factor-_ (TNF-_) in cell culture supernatant and other biological samples. The kit is intended FOR LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.