The dual-color dot-ELISA kit assay is a modification of the T cell ELISPOT assay, one of the most sensitive assays for monitoring ex vivo cellular immune responses at the single cell level. The dot-ELISA kit was developed for the simultaneous detection of two secreted proteins, such as cytokines, which are released by e.g. T cells in response to an antigen. The cell suspensions, used in the assay are usually derived from blood (PBMC).