The TrypLETM Quantitative ELISA Kit (enzyme-linked immunosorbent assay) is a high-sensitivity universal recombinant trypsin-like quantitation kit, capable of accurately identifying various recombinant non-animal sourced trypsin and its analogs. The kit can sensitively, specifically, and accurately detect residues of recombinant trypsin (TrypLE) in intermediates, semi-finished products, and finished products of various biological products. The detection range of this assay kit is 0.1-16 ng/mL, with a optimized quantitative limit of 0.1 ng/mL.