This kit uses Blue, our chromogenic HRP substrate that is much more sensitive to both H2O2 and peroxidase than other chromogenic peroxidase substrates such as TMB, ABTS, OPD and K-Blue. Blue generates a highly absorptive material that has maximum absorption of 664 nm. This near infrared absorption minimizes the background absorption often caused by the auto-absorption of biological samples. The kit provides an optimized mix and read assay protocol that is compatible with HTS liquid handling instruments. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by an absorbance microplate reader at 664?5 nm.